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1. Which compound is used for chemical analysis of living tissues to separate acid-soluble and acid-insoluble fractions?
A. Trichloroacetic acid (CCl3COOH)
B. Hydrochloric acid (HCl)
C. Acetic acid (CH3COOH)
D. Sulphuric acid (H2SO4)
Answer: A. Trichloroacetic acid (CCl3COOH)
Explanation: Grinding living tissue in trichloroacetic acid yields a filtrate (acid-soluble pool) and a retentate (acid-insoluble fraction).
2. Lipids are found in the acid-insoluble fraction despite having a low molecular weight because they:
A. Form membrane vesicles that do not pass through filters
B. Are giant polymeric networks
C. Are strictly macro-biomolecules
D. Covalently bond directly to proteins
Answer: A. Form membrane vesicles that do not pass through filters
Explanation: Lipids form cellular membrane vesicles during homogenization, retaining them in the acid-insoluble retentate, though they are not true macromolecules.
3. Amino acids are termed substituted methanes because four substituent groups occupy the four valency positions of the:
A. Alpha-carbon
B. Beta-carbon
C. Carboxyl carbon
D. Amino nitrogen
Answer: A. Alpha-carbon
Explanation: An amino acid has hydrogen, carboxyl group, amino group, and a variable R-group attached to the central alpha-carbon.
4. Which bond links individual amino acid monomers together to form a polypeptide chain?
A. Peptide bond
B. Glycosidic bond
C. Phosphodiester bond
D. Hydrogen bond
Answer: A. Peptide bond
Explanation: A peptide bond (-CO-NH-) forms when the carboxyl group of one amino acid reacts with the amino group of another with loss of water.
5. Starch gives a characteristic blue color with iodine because:
A. Helical secondary structures of starch trap iodine molecules
B. Iodine oxidizes starch to glucose
C. Starch is a reducing disaccharide
D. Cellulose and starch form complex coacervates
Answer: A. Helical secondary structures of starch trap iodine molecules
Explanation: Starch forms secondary helical structures that hold I2 molecules in their loops. Cellulose lacks complex helices and cannot hold I2.
6. What type of bond connects adjacent nucleotides along a single strand of nucleic acid?
A. 3'-5' Phosphodiester bond
B. Peptide bond
C. Glycosidic bond
D. Ester bond
Answer: A. 3'-5' Phosphodiester bond
Explanation: Nucleotides join sequentially when a phosphate connects the 3'-carbon of one sugar to the 5'-carbon of the next sugar.
7. The primary protein structure represents:
A. The linear sequence of amino acids in a polypeptide chain
B. Local folding into alpha-helices or beta-pleated sheets
C. Three-dimensional folding into a functional active unit
D. Multi-subunit quaternary protein complexes
Answer: A. The linear sequence of amino acids in a polypeptide chain
Explanation: Primary structure specifies the order and sequence of amino acids, identifying N-terminal and C-terminal ends.
8. The most abundant protein in the whole of the biosphere is:
A. RuBisCO
B. Collagen
C. Insulin
D. Hemoglobin
Answer: A. RuBisCO
Explanation: Ribulose bisphosphate carboxylase-oxygenase (RuBisCO) is the most abundant protein in the entire biosphere; collagen is most abundant in animals.
9. Enzymes increase reaction rates by lowering which energy barrier?
A. Activation energy
B. Free energy of reactants
C. Kinetic energy of products
D. Potential energy of substrate
Answer: A. Activation energy
Explanation: Enzymes decrease the activation energy required to convert substrate into the high-energy transition state, speeding up product formation.
10. Inhibition of succinic dehydrogenase by malonate due to structural similarity with succinate is an example of:
A. Competitive inhibition
B. Non-competitive inhibition
C. Allosteric feedback regulation
D. Irreversible denaturation
Answer: A. Competitive inhibition
Explanation: Malonate closely resembles succinate structurally, competing for the active site of succinic dehydrogenase, exemplifying competitive inhibition.
11. Which of the following is an essential aromatic amino acid?
A. Phenylalanine
B. Glycine
C. Alanine
D. Valine
Answer: A. Phenylalanine
Explanation: Tyrosine, phenylalanine, and tryptophan are aromatic amino acids. Phenylalanine is an essential aromatic amino acid.
12. Lecithin is a nitrogenous phospholipid commonly found in cell membranes. It belongs to which class of lipids?
A. Phospholipids
B. Simple fats
C. Steroids
D. Waxes
Answer: A. Phospholipids
Explanation: Lecithin is a glycerophospholipid containing a choline head group attached to a phosphate group, abundant in plasma membranes.
13. The simplest amino acid which lacks a chiral carbon center (optically inactive) is:
A. Glycine
B. Alanine
C. Serine
D. Glutamic acid
Answer: A. Glycine
Explanation: In glycine, the variable R-group is a hydrogen atom, making the alpha-carbon symmetric and optically inactive.
14. The chemical bond present between sugar and nitrogenous base in a nucleoside is:
A. N-glycosidic bond
B. Phosphoester bond
C. Peptide bond
D. Hydrogen bond
Answer: A. N-glycosidic bond
Explanation: Nitrogenous base is linked to the 1'-carbon of pentose sugar via an N-glycosidic linkage to form a nucleoside.
15. Glycogen, the storage polysaccharide in animals, is a branched polymer of:
A. Glucose
B. Fructose
C. Galactose
D. Ribose
Answer: A. Glucose
Explanation: Glycogen is a highly branched homopolymer composed of alpha-D-glucose units stored in liver and muscle cells.
16. Non-protein constituent organic component tightly bound to the apoenzyme to make it catalytically active is called a:
A. Prosthetic group
B. Coenzyme
C. Metal ion activator
D. Isoenzyme
Answer: A. Prosthetic group
Explanation: Prosthetic groups are organic cofactors tightly bound to the protein apoenzyme (e.g., Haem in peroxidase/catalase).
17. Which purine nitrogenous bases are present in both DNA and RNA?
A. Adenine and Guanine
B. Cytosine and Thymine
C. Adenine and Uracil
D. Guanine and Cytosine
Answer: A. Adenine and Guanine
Explanation: Purines (Adenine and Guanine) are double-ring nitrogen bases common to both DNA and RNA.
18. The exoskeleton of arthropods and fungal cell walls are composed of a complex polysaccharide named:
A. Chitin
B. Cellulose
C. Peptidoglycan
D. Pectin
Answer: A. Chitin
Explanation: Chitin is a homopolymer of N-acetylglucosamine (NAG) forming arthropod exoskeletons and fungal cell walls.
19. Zinc acts as a co-factor for which catalytic enzyme?
A. Carboxypeptidase
B. Catalase
C. Peroxidase
D. Dehydrogenase
Answer: A. Carboxypeptidase
Explanation: Zinc is an essential metallic co-factor required for the proteolytic enzyme carboxypeptidase.
20. In B-DNA double helix structure, the pitch of the helix per turn is:
A. 3.4 nm (34 Å)
B. 0.34 nm (3.4 Å)
C. 2.0 nm (20 Å)
D. 34 nm (340 Å)
Answer: A. 3.4 nm (34 Å)
Explanation: B-DNA pitch is 3.4 nm per complete 360-degree helical turn containing 10 base pairs (0.34 nm per base pair distance).
21. Secondary metabolites that act as toxins include:
A. Abrin and Ricin
B. Morphine and Codeine
C. Concanavalin A
D. Vinblastine and Curcumin
Answer: A. Abrin and Ricin
Explanation: Abrin (from Abrus precatorius) and Ricin (from Ricinus communis) are classified under plant-derived secondary metabolite toxins.
22. Concanavalin A belongs to which group of plant secondary metabolites?
A. Lectins
B. Alkaloids
C. Essential oils
D. Polymeric substances
Answer: A. Lectins
Explanation: Concanavalin A is a carbohydrate-binding protein classified as a lectin under secondary metabolites.
23. Saturated fatty acids differ from unsaturated fatty acids in having:
A. Only single carbon-carbon bonds in their hydrocarbon chain
B. One or more double bonds (-C=C-)
C. Ring structures with double bonds
D. Esterified glycerol groups
Answer: A. Only single carbon-carbon bonds in their hydrocarbon chain
Explanation: Saturated fatty acids (like palmitic acid) contain no double bonds between carbon atoms in the acyl chain.
24. Total number of carbon atoms present in palmitic acid including the carboxyl carbon is:
A. 16
B. 20
C. 18
D. 15
Answer: A. 16
Explanation: Palmitic acid has 16 carbons (CH3-(CH2)14-COOH), whereas arachidonic acid has 20 carbons.
25. Isoenzymes are enzymes that:
A. Differ in amino acid sequence but catalyze the same chemical reaction
B. Have identical primary structures but different substrates
C. Require identical non-protein cofactors
D. Function only at sub-zero temperatures
Answer: A. Differ in amino acid sequence but catalyze the same chemical reaction
Explanation: Isoenzymes (isozymes) are distinct molecular forms of an enzyme with different physical properties that catalyze the same biochemical reaction.
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